FloodLAMP Rapid Molecular Test Training Program

Introduction to the FloodLAMP test training program for our QuickColor test - a molecular LAMP reaction suitable for low cost, flexible disease screening.

Safety and contamination protocols are discussed, including PPE, compliance with safety regulations, handling RNA, avoiding various types of contamination, such as RNAse, positive control contamination, sample cross-contamination, and amplicon contamination.

Shows the preparation of the 1X inactivation saline solution (IXSSS). This reagent is added to the sample which in then heated, breaking open the virus and releasing the RNA for subsequent amplification.

Shows adding the inactivation solution to the sample tubes, followed by heating the samples, then allowing to cool at room temperature.

Preparing a reaction mix for 48 reactions, including handling master mix and PGS, and logging procedures as per SOPs. Provides detailed instructions on preparing strip tubes, avoiding contamination, mixing and measuring components correctly, and storing the prepared mix in the freezer for future use.

Handling of reaction strips, including tips for checking seals and avoiding air gaps, and emphasizes the importance of timely freezer storage and the use of a cold block for multiple strips to maintain optimal temperatures during the preparation process.

Shows the process of adding samples to strip tubes, including organization and labeling, careful handling of controls and samples to prevent cross-contamination, and the importance of following protocol steps for preparing and sealing the tubes for amplification, with additional emphasis on glove changes.

Shows the sample intake step which involves scanning the QR codes on the sample tubes with the FloodLAMP Mobile App.

Once the amplification reaction heat step is finished, the samples are put into a lightbox and a photo is taken with a smartphone. A change in the liquid color from pink to yellow indicates the target (SARS-CoV-2 virus) is present.

Shows the completion of a lab run, including updating results in an app, logging the run with a form link, and uploading a photo of the results.

Shows the process of preparing and handling numerous sample tubes for testing, emphasizing the need for careful organization, checking and securing lids, and efficiently dispensing inactivation solution. Practical tips are demonstrated.

Shows the steps of inactivation on many samples, including safely removing equipment from the table, vortexing samples together in a rack, cleanliness protocols, placing the samples in a water bath with caution to avoid steam burns.

Shows preparation steps for running an entire plate of up to 94 samples. Emphasizes the use of foil, plastic bags, cold block, and plate seals.

Procedure for processing a 96 well reaction plate, including inactivation, adding controls, and running amplification reaction.

Details the process of organizing and cleaning a laboratory workspace, including taping off areas, using bleach and ethanol for cleaning surfaces and items like pens and phones, and the importance of maintaining a sanitized environment for testing.

Thoroughly cleaning of pipettes and their boxes using bleach and ethanol, emphasizing the importance of cleanliness and proper drying. Also includes organizing the cleaned equipment on the table.

Shows the setup process for a dispenser, including organizing components, handling the 1X inactivation solution, ensuring cleanliness with a plastic bag, managing cords, and conducting a system test to confirm the dispenser's readiness for use.